Journal: Nature communications

Article Title: Trimannose-coupled antimiR-21 for macrophage-targeted inhalation treatment of acute inflammatory lung damage.

doi: 10.1038/s41467-023-40185-1

Figure Lengend Snippet: Fig. 1 | Identification of miR-21 in macrophages as therapeutic target for acute inflammatory lung disease. a Overview of the study design. b Scatter plot of differentially expressed microRNAs in bleomycin lungs compared to control lungs. Each dot represents one microRNA. Differentially expressed microRNAs are high- lighted in red (FDR < 0.05, −0.75 < log2FC > 0.75, mean normalised counts > 1000). Control n = 3, Bleomycin n = 3. c Scatter plot of differentially expressed microRNAs in COVID-19 lungs compared to control lungs. Each dot represents one microRNA. Differentially expressed microRNAs are highlighted in red (FDR < 0.05, −0.75 < log2FC > 0.75, mean normalised counts > 1000); Control n = 12, COVID-19 n = 14. d Lollipop graph represents the top 10 expressed microRNAs in (left) mouse and (right) human lung macrophages (n = 3-4). e Representative staining for macro- phage marker CD68 in bleomycin-induced mouse lungs (n(PBS) = 3, n(bleo) = 3) and COVID-19 patients (n(COVID-19) = 3, n(Control) = 3). Scale bar represents

Article Snippet: Mice lung cryosections (5 μm) were fixed with 4% paraformaldehyde (PFA) and stained with rat anti-mouse CD68 (FA-11, 1:50 dilution in 3% goat serum/PBS, BIO-RAD) or rabbit anti-mouse MRC1 (1:100 dilution in 3% goat serum/PBS, abcam) at 4 °C overnight.

Techniques: Control, Staining, Marker

Journal: Andrology

Article Title: Human efferent ductules and epididymis display unique cell lineages with motile and primary cilia

doi: 10.1111/andr.13732

Figure Lengend Snippet: Identity, sources, and working dilution of antibodies used in this study .

Article Snippet: CD68 , Mouse , EDTA buffer , 1/6000 , 66231‐2‐Ig , Proteintech.

Techniques:

Journal: Journal of Biological Chemistry

Article Title: Apolipoprotein A-I Modulates Regulatory T Cells in Autoimmune LDLr−/−, ApoA-I−/− Mice

doi: 10.1074/jbc.m110.134130

Figure Lengend Snippet: FIGURE 7. ApoA-I treatment reduces immune cell infiltrate into the skin. Panels A–D show H&E staining of skin sections from each of the four study groups; panels E–H show immunofluorescence staining for CD68 (macrophages);panelsI–LshowimmunofluorescencestainingforCD11c(dendriticcells).PanelsA,E,andIare skin sections from 12-week diet-fed SKO mice; panels B, F, and J are skin sections from 6-week diet-fed DKO mice; panels C, G, and K are skin sections from 12-week diet-fed DKO mice; panels D, H, and L are skin sections from 12-week diet-fed DKO mice treated for 6 weeks with apoA-I. Sections shown are representative of at least four sections per animal with at least 4–6 different animals per genotype and treatment group.

Article Snippet: Next, sections were incubated in their respective secondary antibody goat anti-rat IgG CD68 Cy3 (Rockland) at 1:300 or biotin-conjugated CD3e, CD11c mouse anti-hamster IgG (Pharmingen) for 45 min at room temperature.

Techniques: Staining, Immunofluorescence

Journal: bioRxiv

Article Title: Complement C5a receptor 1 blockade reverses cognitive deficits following cranial radiation therapy for brain cancer

doi: 10.1101/2024.07.02.601806

Figure Lengend Snippet: (A) Dual immunofluorescence staining, confocal microscopy, and 3D algorithm-based, volumetric quantification for the microglial activation (CD68-IBA1 immunoreactivity, magenta-green) in the WT mice show cranial irradiation (9 Gy + Vehicle)-induced elevated CD68 expression by the IBA1 + microglia in the hippocampus ( dh , dentate hilus; dg , dentate gyrus) compared to 0 Gy+Vehicle. (B) A separate CD68-DAPI channel (magenta-blue) is shown for each group. (C) C5aR inhibition (PMX205) in WT mice did not elevate CD68 immunoreactivity in the irradiated brain (9 Gy+PMX205), indicating prevention of inflammation. (D-E) Volumetric quantification for the microglial C5aR1 expression (C5aR1-IBA1 immunoreactivity, magenta-green) showed a radiation-induced increase in the microglial-C5aR1 in the WT hippocampal dg and dh compared to unirradiated controls. PMX205 treatment reduced microglial-C5aR1 expression compared to the 9 Gy mice +vehicle ( F ). (G) Analysis of astrogliosis (GFAP-C3d) showed radiation-induced elevation in GFAP + astrocytic surface co-labeled with complement C3d (GFAP surface rendering, magenta, and C3d spot rendering, green) in the 9 Gy+Vehicle compared to 0 Gy+Vehicle or 0 Gy+PMX205 groups in the hippocampal dg . 9 Gy mice receiving C5aR inhibitor (PMX205) did not show elevated GFAP-C3d immunoreactivity, indicating reduced astrogliosis (H) . Mean±SEM ( N =6-8 per group). Scale bars, 50 µm ( A-B, D-E ) and 10 µm ( G ).

Article Snippet: This included 3% normal goat serum, NGS with 1% Bovine Serum Albumin or BSA in Tris-A buffer for synaptophysin; 4% BSA in Tris-A buffer for PSD95; 3% NGS in Tris-A buffer for CD88 (C5aR1) and CD68/IBA1 markers (NGS, Jackson ImmunoResearch Cat. 005-000-121; BSA, Sigma-Aldrich Cat. 05470) at rt for 45 minutes.

Techniques: Immunofluorescence, Staining, Confocal Microscopy, Activation Assay, Irradiation, Expressing, Inhibition, Labeling